Future Solutions to HIV AIDS :: Biology Disease

Introduction to HIV What is HIV? First Things First: What is HIV? The Human Immunodeficiency Virus (HIV) is one of the most evasive viruses that the medical world has to experience. We use the word evasive because HIV targets the CD4 cells (T-helper cells), which are the exact cells that are used to protect your body against diseases. Once HIV enters the body, it will infect the CD4 cells and begin to use those cells as its own â€Å"HIV factory.† HIV will therefore begin to destroy your immune system leaving you susceptible to opportunistic diseases. HIV targets your immune system... What does HIV infect and how? HIV affects your immune system. Your immune system can be thought of as your body’s defense line! In which, you have millions of 'soldiers' (T-helper cells) helping you ward off almost any foreign invaders. Now, it is important to note that just like in a army defense line, your body also has different levels of protection. Introduction to HIV Cont. Your skin is the first line of defense... †¢ First line of defense: the skin o Your skin is an amazing organ, which helps you ward off invaders from the outside. Special cells called Dendritic cells and macrophages are white blood cells that try to fight off infection before it becomes a major problem. T-Cells of the humoral immune response are the second line of defense... †¢ Second line of defense: T-Cells o Once a virus has invaded the body, T-Cells (CD4 cells) relay signals to the rest of the immune system, which causes the immune system to activate by sending out the â€Å"reinforcements.† These T-killer cells (CD8 cells) do exactly what the name suggests, they â€Å"kill† almost any foreign invader, through apoptosis or programmed cell death. Other CD4 activated cells are part of your body's line of defenses... †¢ Other Cells activated by CD4 Cells o B-Cells- Once activated, B-cells / Plasma cells begin producing antibodies against a virus o Natural Killer Cells (NK)- Help your body by destroying infected cells Introduction to HIV Cont. All viruses must be able to bind to a cell in order to enter it. In the case of HIV, the virus has a cell membrane with embedded viral proteins that enables it to do so, but this works only when it binds with two specific receptors. One is called the main receptor, and the other a coreceptor. There are two types of HIV; M-tropic and T-tropic. The M-tropic variant targets macrophages while the T-tropic variant targets the T cells. Future Solutions to HIV AIDS :: Biology Disease Introduction to HIV What is HIV? First Things First: What is HIV? The Human Immunodeficiency Virus (HIV) is one of the most evasive viruses that the medical world has to experience. We use the word evasive because HIV targets the CD4 cells (T-helper cells), which are the exact cells that are used to protect your body against diseases. Once HIV enters the body, it will infect the CD4 cells and begin to use those cells as its own â€Å"HIV factory.† HIV will therefore begin to destroy your immune system leaving you susceptible to opportunistic diseases. HIV targets your immune system... What does HIV infect and how? HIV affects your immune system. Your immune system can be thought of as your body’s defense line! In which, you have millions of 'soldiers' (T-helper cells) helping you ward off almost any foreign invaders. Now, it is important to note that just like in a army defense line, your body also has different levels of protection. Introduction to HIV Cont. Your skin is the first line of defense... †¢ First line of defense: the skin o Your skin is an amazing organ, which helps you ward off invaders from the outside. Special cells called Dendritic cells and macrophages are white blood cells that try to fight off infection before it becomes a major problem. T-Cells of the humoral immune response are the second line of defense... †¢ Second line of defense: T-Cells o Once a virus has invaded the body, T-Cells (CD4 cells) relay signals to the rest of the immune system, which causes the immune system to activate by sending out the â€Å"reinforcements.† These T-killer cells (CD8 cells) do exactly what the name suggests, they â€Å"kill† almost any foreign invader, through apoptosis or programmed cell death. Other CD4 activated cells are part of your body's line of defenses... †¢ Other Cells activated by CD4 Cells o B-Cells- Once activated, B-cells / Plasma cells begin producing antibodies against a virus o Natural Killer Cells (NK)- Help your body by destroying infected cells Introduction to HIV Cont. All viruses must be able to bind to a cell in order to enter it. In the case of HIV, the virus has a cell membrane with embedded viral proteins that enables it to do so, but this works only when it binds with two specific receptors. One is called the main receptor, and the other a coreceptor. There are two types of HIV; M-tropic and T-tropic. The M-tropic variant targets macrophages while the T-tropic variant targets the T cells.

Tissue Culture Technique for Callus Induction

Sarhad J. Agric. Vol. 23, No. 1, 2007 * Department of Plant Breeding and Genetics, NWFP Agricultural University, Peshawar – Pakistan. ** Agricultural Biotechnology Institute, National Agricultural Research Center, Islamabad – Pakistan. TISSUE CULTURE TECHNIQUES FOR CALLUS INDUCTION IN RICE Hidayat Ullah*, Iltaf Ullah*, Sultan Akbar Jadoon* and Hamid Rashid** ABSTRACT Seeds of two rice varieties Basmati-370 and Basmati-385 were evaluated for invitro callus induction at Agricultural Biotechnology Institute in National Agricultural Research Center (NARC), Islamabad during 2001-02, using M.S and N6 media supplemented with 2, 4-D (2,4-Dichlorophenoxi acetic acid) and BAP (6-benzylaminopurine) at @ 2. 0, 2. 5 and 0. 1, 0. 5 mgl-1 respectively. Objective of our study is to know the actual procedure for seed sterilization and to provide a well-adopted technique for callus induction in these varieties by using M. S and N6 media along with different concentrations of growth hormo nes. Seeds were used as explant source and to avoid chance of contamination sodium hypochlorite and ethanol at the rate of 50% and 70% were used respectively.Best response toward callus induction were observed for Bas-385 on both MS and N6 media however N6 media was proved to be best. Calli obtained from Bas-385 were friable and vigorous as compare to Bas-370. Bas-370 respond moderately on MS and N6 media at various combinations of 2,4-D and BAP. Bas-370 on MS media supplemented with 2,4-D and BAP @ 2. 0 and 0. 1 mgl-1 gave good performance towards callus induction. Over all results indicated that best callus were induced on MS and N6 when supplemented with 2,4-D and BAP @2. 0 and 0. 0 mgl-1 for variety Bas-385. INTRODUCTION In Pakistan rice is the second leading crop after wheat.In 2000-2001, the world annual production of rice was 598 million tones (FAO 2002). Asia is the largest producer of rice, with Bangladesh, China, Philippines, Thailand and Vietnams as the leading rice produ cing nations. In Pakistan rice covered an area of 2. 52 million ha, with the production of 5. 16 million tones in 1999-2000. It also play important role in our national economy. During 1999-2000 about 1. 92 million tones of rice was exported and earn about 465. 8 million US$. Rice is susceptible to a range of diseases and pests, which annually destroy about 55 percent of rice crops. The most common diseases are caused by he fungi sheath blight and rice blast, and the stalk borer is a common insect pest. Rice is composed of essential food components, therefore more than two billions people in the globe depend on rice for more than half of the proteins and calories they consume (Khan et al. 2000). Due to its increasing importance in nutrition and economy, it is now felt that new varieties of rice, having good agronomic characters, should be evolved. Crop improvement through tissue culture techniques is easier and more often in use as compared to conventional plant breeding (Yamada, 19 86). Somaclonal variations commonly ppear after tissue culture, which involve a callus stage (Larkin and Scow Croft 1981). Callus is undifferentiated mass of rapidly proliferating cells, can be obtained by culturing explants source (seed, node, bud, leaves, meristem and root tips etc) on nutrient medium containing specific growth regulators along with a standard recipe of chemicals. Rashid et al. (2000) studied that rice seeds have more potential for callogenesis as compared to node or tip. Successful callus induction from rice seed has been reported by several researchers (Gonalz 2000; Navraj et al. 1999; Marrassi 1996; Valdez et al. 1997; Xie et al. 995). But an improved method for callogenesis was reported by Rashid et al. 2000). The present study is based on tissue culture techniques carried out in ABI laboratory for callus induction in rice varieties viz. Basmati-370 and Basmati-385. Two types of basal media M. S (Murashige and Skoog 1962) and N6 (Nitsch and Nitsch, 1969) suppl emented with 2,4-D (2,4- dichlorophenoxyacetic acid) alone or in combination with different concentrations of BAP (6-benzylaminopurine) were used for callus induction. Hidayat Ullah, et al. Tissue culture techniques for callus induction in rice†¦. 82 MATERIALS AND METHODSThe research work for callus induction in rice was conducted at the tissue culture laboratory of Agricultural Biotechnology Institute in National Agricultural Research Center (NARC), Islamabad, during 2001-02. The procedure of this research work has been divided in the following four main categories. Surface Sterilization of Rice Seeds Seeds of Basmati-370 and Basmati-385 were provided by rice programmed, Agricultural Biotechnology Institute (ABI), National Agricultural Research Center Islamabad, and it was taken as explant source for callus induction. Healthy and mature seeds were selected by hysical appearance and they were dehusked manually. Seeds were first washed with detergent and then rinsed three times with simple tap water. For surface sterilization of seeds Clorox (5. 25% sodium hypochlorite) and ethanol was applied. After the applications of Clorox and ethanol seeds were rinsed thrice with autoclaved distilled water. Finally the seeds were dried with autoclaved filter paper, and they were shifted carefully to the culture room of Agricultural Biotechnology Institute. Basal Media Preparation M. S and N6 basal media were used for callus initiation. These media were prepared according to he ingredients (Table I). The exact amount of nutrients was dissolved in the distilled water. Two types of growth regulators 2,4-D alone or in combination with BAP was used for callus induction. 2,4-D @ 2. 0 and 2. 5 mg l-1 and BAP @ 0. 0, 0. 1, 0. 5 mg l-1 respectively was added in the media. Sucrose at the rate of 3% and agar at the rate of 0. 7% was also added in the media. PH of the media was adjusted at 5. 78-5. 80 with the help of PH-meter. M. S. and N6 media were poured into the test tubes, it was plugged properly and autoclaved at 20 lbs pressure for 15 minutes in the autoclave machine.Inoculation of Sterilized Seeds The most important step in tissue culture technique is the inoculation of seeds. This operation was performed in the laminar flow cabinet at the culture room of ABI. Before the operation surface sterilization of the laminar flow unit was carried by UV-light for two minutes. After that hands were disinfected with 75% ethanol to prevent chance of contamination. Dried seeds were then inoculated into test tubes under aseptic condition in laminar flow unit. To minimize chance of infection the instruments were dipped in disinfectant after every operation. Growth ChamberInoculated cultures were incubated at 25 ±3? C under the influence of 2000-lux light intensity for 16 hours photoperiod. Callus induction of rice seeds were observed after 21- days. The data for callus induction frequency were recorded for two rice varieties on two different media with differen t concentrations of growth regulators. Table I Composition of MS and N6 media Macronutrients KNO3 1900 2830 NH4NO3 1650 0 CaCl2. 2H2O 440 166 MgSO4 370 90. 37 KH2PO4 170 400 (NH4)2 SO4 — 463 Micronutrients MnSO4. H2O 16. 9 3. 33 H3BO3 6. 2 1. 6 KI 0. 83 0. 80 ZnSO4. 4H2O 8. 6 — ZnSO4. 7H2O — 1. 5 NaMoO4. 2H2O 0. 5 — CuSO4. 5H2O 0. 025 — CoCl2. 6H2O 0. 025 — Iron source FeSO4. 7H2O 27. 85 27. 85 NaEDTA 37. 25 37. 25 Vitamins Myoinositol 100 — Nicotinic Acid 0. 5 0. 5 Pyridoxine HCl 0. 5 0. 5 Thiamine HCl 0. 1 1. 0 Glycine 2. 0 — Sucrose 30g 30g Agar 7g 7g RESULTS AND DISCUSSION Results obtained from tissue culture techniques of Bas-370 and Bas-385 performed in ABI laboratory and repeated three times from 10th February to Sarhad J. Agric. Vol. 23, No. 1, 2007 83 October 2001. Chance of contamination was much low when both the varieties were treated with 50% Clorox and 70% ethanol. Oono (1981) also used eeds as explants source for rice callus induction. The main objective of our study is to know the actual procedure for seed sterilization and callogenesis in these cultivars. The potential of both varieties for callus induction was observed significantly different on M. S and N6 media at different concentrations of growth regulators. It is noteworthy that N6 and M. S media, which proved to be optimum for the growth of callus, were unable to support cell growth in liquid media. Callus induced from both the varieties were different at various level of 2,4-D and BAP. Maximum callus formation (62. 5%) was recorded or Bas-385, followed by Bas-370 (55. 55%) when seeds were cultured on MS medium supplemented with 2,[email  protected] 0 mgl-1 and 2mgl-1 2,4-D+0. 1mgl-1 BAP respectively (Table II and IV). Lowest callus of 41. 66 % was observed each for Bas-370 and Bas-385 when MS medium is supplemented with 2. 5 and 0. 5 mgl-1 of 2,4-D and BAP respectively. It is cleared that potential of Bas-385 towards callogenesi s was higher than Bas-370. Averaged across MS media callus induction frequency was 48. 14 % for BAS-370 vs 53. 22 % for BAS-385 (Fig 2). This is equivalent to the net reduction of 5. 08 % for BAS-370. Growth regulator 2,4-D at he rate of 2mgl-1 was suggested and proved to be best for callus induction in both the varieties. Using N6 media callus induction frequency ranged from 20. 83 to 68. 05% for both the varieties. When 2,[email  protected] was supplemented with N6 media, produced highest and good quality callus from Bas-385 (68. 05%) and minimum (20. 83%) from Bas-370 (Table III and IV). This result indicated positive correlation between 2,4-D and Bas-385 and negative for Bas-370. Average across N6 media callus induction frequency was 23. 6 % for Bas-370 vs 60. 64 % for Bas-385 (Fig. 3). This is equivalent to the net reduction of 7. 2 % for BAS- 370. Calli obtained from both the varieties were friable, granular, and yellow in color. So it was proved that N6 media is better for callus induction of rice as compared to M. S medium. Overall result of BAS-385 was best on various concentrations of 2,4-D and BAP on both, MS and N6 media. Callus form Bas-385 was healthy and more fleshy as compare to Bas-370 (Figure 1). The active division of cells was more prominent in the callus of Bas-385, that’s why it looks bulky. Secondly Bas-385 had more capacity for producing callus, especially when N6 media supplemented with 2,4- [email  protected]Agronomic features of Bas-385 are also better than Bas-370 because it is developed through hybridization between Bas-370 and TN-1. Callus induction must be dependent on the genetic potentials of the variety and secondly the combination of hormones at different concentration. Callus induction is the best way to create somaclonal variations in crop plants. Variations are the basis for improvement and some time this variation is heritable. Oono (1981) cultured seed explants of rice varieties and observed variation for cert ain agronomic characters that are also heritable. Once cultured techniques were stablished in rice, it become possible to apply them for callus regeneration and transformation. (b) Calli obtained from Bas-385 (a) Calli Obtained form Bas-370 Hidayat Ullah, et al. Tissue culture techniques for callus induction in rice†¦. 84 Table II Percentage callus induction frequency and contamination in Bas-370 on M. S Medium Treatments Sterilizing agents Clorox time/Ethano l time (min) Hormone in mg L-1 # Of inoculated test tubes % Contamination % Callus induction frequency % Clorox % Ethanol 2,4-D BAP 1 50 70 20/1. 0 2. 0 0. 0 72 58. 33% 20. 83% 2 50 70 20/2. 0 2. 0 0. 1 72 73. 61% 22. 22% 50 70 20/4. 0 2. 5 0. 5 72 52. 77% 27. 77% Table III Percentage callus induction frequency and contamination in Bas-370 on N6 Medium. Treatments Sterilizing agents Clorox time/Ethano l time (min) Hormone used in mg L-1 # Of inoculated test tubes % Contami nation % Callus induction frequency % Clorox % Eth anol 2,4-D BAP % Clorox 1 50 70 20/1. 0 2. 0 0. 0 72 1 50 2 50 70 20/2. 0 2. 0 0. 1 72 2 50 3 50 70 20/4. 0 2. 5 0. 5 72 3 50 Table IV Percentage callus induction frequency and contamination in Bas-385 on M. S Medium. Treatments Sterilizing agents Clorox /Ethanol time (min) Hormone used in mg L-1 Of inoculated test tubes % Contami nation % Callus induction % Clorox % Ethanol 2,4-D BAP % Clorox 1 50 70 20/1. 0 2. 0 0. 0 72 1 50 2 50 70 20/2. 0 2. 0 0. 1 72 2 50 3 50 70 20/4. 0 2. 5 0. 5 72 3 50 Table V. Percentage callus induction frequency and contamination in Bas-385 on N6 Medium Treatments Sterilizing agents Clorox/Etha nol time (min) Hormone used in mg L-1 # Of inoculated test tubes % Contami nation % Callus induction frequency % Clorox % Ethanol 2,4-D BAP % Clorox 1 50 70 20/1. 0 2. 0 0. 0 72 1 50 2 50 70 20/2. 0 2. 0 0. 1 72 2 50 3 50 70 20/4. 0 2. 5 0. 5 72 3 50 Sarhad J. Agric. Vol. 3, No. 1, 2007 85 20. 83 62. 5 22. 22 55. 5 27. 77 41. 66 0 20 40 60 80 Bas-370 Bas-385 Variet ies Callus induction freq (%) 2,4-D&[email  protected] 0&0. 0 2,4-D&[email  protected] 0&0. 1 2,4-D&[email  protected] 5&0. 5 47. 22 68. 05 55. 55 61. 11 41. 66 52. 77 0 10 20 30 40 50 60 70 80 Bas-370 Bas-385 Varieties Callus induction freq (%) 2,4-D&[email  protected] 0&0. 0 2,4-D&[email  protected] 0&0. 1 2,4-D&[email  protected] 5&0. 5 Fig. 2. Callus induction frequency (%) of Bas-370 and Bas-385 on M. S media at different level of 2,4-D and BAP. Fig. 3. Callus induction frequency (%) of Bas-370 and Bas-385 on N6 media at different level of 2,4-D and BAP. Hidayat Ullah, et al.Tissue culture techniques for callus induction in rice†¦. 86 CONCLUSION AND RECOMMENDATION Infections of the callus is main problem in tissue culture technique so we should adopt the following precautions. 1. We should sterilize the seeds in 50% hypochlorite solution for 20 minutes and all the apparatus like flasks, petri plates, blades and forceps etc. should be disinfectant with 70% ethan ol. Media should be prepared accurately with respect to concentrations and PH of the media must be maintained properly. Exact amount of growth hormones must be added. 2. Seeds of Bas-370 on MS media with combination 2. and 0. 1mgl-1 should be used to get maximum callus, while hormonal combination 2,4-D and BAP @2. 0 and 0. 0 mg l-1 should be used for better callus induction of Bas-385 on N6 media. Bas-385 is an improved version of Bas-370 because it is developed through hybridization between Bas-370 and TN-1. That’s why it is more responsive towards callus as will as high doses of fertilizers. Over all results indicated that Bas- 385 having more potentials and suitable for further genetic studies. REFERENCES FAO. 2002 Food Outlook Bullet. No: 1,FAO, Rome. Gonalz, M. C. 2000. Effects of different growth egulators on in vitro culture of rice cultivors. Tropicales. 21(1): 27-28. Khan, J. A. , F. F. Jamil and M. A. Gill. 2000. Screnning of rice varieties against bakanae and bacte rial leaf blight. Pak. J. Phytopath. 12(1): 6-11. Larkin, P. J. and W. R. Scowcraft. 1981. Somaclonal variation a novel source of variability from cell cultures of plant improvement. Theor. Appld. Genet. 60:197-214. Marasi, M. A. , O. A. Bovo, A. Socchi and L. A. Mrongiski. 1996. Cytokining in the callus induction medium for plant regeneration of rice. Phyton Int, J. Exp. Bot. 59(1-2): 155-160. Murashige, T. and F. Skoog. 1962.Arevised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497. Navraj, K. , M. S. Gill, G. Raman, T. S. Bharaj, S. S. Gosal, N. Kaur and R. Gill. 1999. Factors enhancing somatic embryogenesis and high frequency plant regeneration in rice. Crop Improv. 26(1): 23-27. Nitsch, J. P. and C. Nitsch. 1969. Haploid plants from pollen grains. Sci. 163: 85-87. Oono, K. 1981. In vitro methods applied to rice in plant tissue culture. T. Athorpe, ed. Acad. Press, New York. pp. 273-298. Rashid, H. , A. Toriyama, K. Qurashi, Hinta a nd K. A. Malik. 2000. An improved method for hoot regeneration from calli of Indica rice. Pak. J. Biol. Sci. 3 (12): 2229-2231. Valdez, M. , M. Monoz, J. R. Vega and A. M. Espinoza. 1997. Plant regeneration of Indica rice from mature embryo derived calli. Revistade Biologia Tropical. 44(3): 13-21. Xie, J. H. , M. W. Gao, Q. H. Cai, X. Y. Chens, Y. W. Shen and Z. Q. Liang. 1995 Optimized growth regulators combination in Japonica rice. Plant Cell Tissue and Organ Culture. 42(3): 245-250. Yamada, Y. , T. Q. Yang and D. T. Tang. 1986. Plant regeneration from protoplast derived callus of rice (Oryza sativa L. ). Plant Cell Reports. 4: 85-88

Reflective Account of My Day at Work Essay

Yesterday I started my day at work at 7:30 am as I was rotted for an early shift as a duty manager for that day. I am following three weeks rolling rota with my colleagues from Senior Management Team (SMT). First of my responsibilities determined by Health and Safety at Work Act as well as our own Signing in Book Policy was to sign in the signing in book. This will ensure that it is known that I am in the building. All the staff coming on duty is required to sign in that book so organisation can keep the track of who is on site for example in case of fire: â€Å"Should the fire alarms go off it is the responsibility of the Team Coordinator to bring the Signing In Book to the assembly point for checking† (Signing in Book Policy) After that I went to the team office where I joined handover from night staff to morning team. â€Å"The Purpose of handover is to ensure the continuity and quality of care through good communication† states our Staff Handovers Policy and it is a lso one of the legal requirements (NMS 17.6). Last night was quiet and all Young People had a good night sleep. After making sure that our staff levels are appropriate for the number of Young People (NMS 17.1, The Children’s Home Regulations Act 2001 and Staffing Policy: â€Å"This policy has been created to ensure that each child is supported and taught appropriately and kept safe whilst accommodated within Our Place†.) I carried out my daily checks. This includes of making sure that teams carry out health and safety checks as well as housekeeping checks. This provides prove that our company ensures clean and safe environment for Young People in our service NMS 10. I didn’t find any short falls in the check books so I signed them off and the next piece of documentation I checked was MAR sheets (Medication Administration Record sheets). The Children’s Home Regulations Act 2001 – section 21 gives a very clear guidance for training, administration and record keeping of medication. Once again I didn’t find any faults or discrepancies. After I completed my daily checks I went to my desk in main office to catch up with my emails. After checking and replying to all my emails I started working on staff’s rota for currant month. Once again, as mentioned above (NMS 17.1, The Children’s Home Regulations Act 2001 and Staffing Policy: â€Å"This policy has been created to ensure that each child is supported and taught appropriately and kept safe whilst  accommodated within Our Place†.), it is my responsibility in organisation that home is staffed correctly and all the Young People are getting support required to meet their individual needs. It means booking our own staff for overtime shifts or using external agency if there are any gaps in our rota. Later on for rest of the rest of my day I was interviewing candidates for Support Worker role. Our company has got very thorough Recruitment & Selection Policy that gives a very clear guidance on the recruitment and selection process, starting with the section on â€Å"assessing the need for recruitment† and going thru all the steps of recruitment process in very detail (setting up recruitment file, advertising, sending out application packs, shortlisting candidates for interviews, interviewing, appointing candidates and eventually gaining the reference and DBS checks as part of safer recruitment and safeguarding Young People). Our Recruitment & Selection Policy is based on all relevant legislation (Children’s Act 1989, The Children’s Home Regulations Act 2001, National Minimum Standards, Keeping Children Safe in Education April 2014). In between the interviews I carried on with my electronic correspondence with Local Authorities, Parents or other external professionals and I also had my lunch break. In the afternoon I handed over everything to my colleague (Staff Handovers Policy, NMS 17.6) and I went home signing out in signing in book.

buy custom The Role of Chemical Engineering essay

buy custom The Role of Chemical Engineering essay Green engineering is a phrase used to explain the role of engineering to reduce or eliminate daily carbon emissions. Green engineering is a process through which products and services are designed in order to lower their impact on the environment. This process eliminates the health hazards posed to man and the environment through pollution. The processes used in green engineering should be economically feasible and cost effective when applied to the existing processes of product design. Environmental remediation is a phrase meaning the restoration of a deeply contaminated location or place to a condition that it will not be a threat to life forms or human health. In conventional clean ups, the contaminated site is usually fenced off to prohibit human and animal entry but green engineering designs methods that will clean the air, soil and water in such a site. This paper highlights the role of chemical engineering processes and methods in environmental remediation. Each year, industries across the world generate multi-billion tons of waste. This is a potential risk to all the life on the planet and consequently environmental degradation. In addition to that, recycling and managing that waste is costing enterprises millions of shillings although regulatory measures are in place. From the start of industrialization, laws regarding environmental protection are on the increase, but some have done little to protect the environment against degradation (NRC Board, 2000). Green engineers have resorted to designing systems that will remediate the environment from choking in waste. They have used green chemistry methods like selecting chemicals that are not toxic, have low potential hazards, which are eliminated through the reduction of chemical exposures. Environmental designs try to reduce risk to the natural ecosystems, workers in industries and consumers of the goods. Before designing any process that remediates the environment, green engineers, have to access the risks of a production process to the environment and human life. A risk can be expressed as the role of exposures and hazards. A hazard is defined as anything that produces a detrimental effect on the human environment while exposure is defined as the qualitative assessment of a chemical when it is exposed to the human or animal body, for instance the skin and the respiratory system. It is vital to note that, conventional methods of preventing pollution focus on the reduction of waste while modern methods of green engineering evaluate the risk posed by the chemicals used in production. Using the approach of risk assessment, engineers have been able to design processes that will be economically beneficial and feasible while reducing the risk to the environment. The application of risk concepts has made green engineers to design green processes and products. They have optimized designs with the purpose of reducing environmental impacts. Chemicals that need to eliminated or minimized have been highlighted and industries are educated on their adverse effects. Green engineers have estimated the risk that the current processes pose to the human environment and people, in addition to accessing the recycle channels and feeding processes. Chemical Precipitation Industries and sewage systems emit a lot of water contaminated with heavy metals. In the conventional systems, the water is kept in treatment plants where heavy metals are not removed. Heavy metals like lead, mercury, cobalt may not be removed from effluents through the filtering or precipitation processes. Green engineering is evaluating chemical products and synthesizers used in water treatment. These chemicals may prove to be useful in remediation of wastewater. The methods have to ensure that the precipitates will not deteriorate and discharge heavy metals to the surroundings. The fist method involves the treatment of wastewater using alum and a polymer. These chemicals enable the precipitation of heavy metals in wastewater. In addition to that, they reduce the PH f treated wastewater from about 8.5 and lower it to seven. A Californian Company, ETUS, Inc., manufactures Thio-Red, a commercial product that has been successful in removing heavy metals from sewage and industrial water. This product reacts with heavy metals to form metal sulfides instead of metal thiocarbonates. The other products include TMT-55 and TMT-15, which are very efficient in precipitating copper, lead, and silver from effluents (Henke 7-55). William Oswald, an environmental scientist at Berkeley University, developed the process of treating water using algae photosynthesis in ponds that are openly incorporated. This system is used to treat sewage in almost all countries in the world (Earth Sciences Division, n.d). This system involves the design of systems that will use microbial genomics in relation with biofuels and recovery of hydrocarbons emitted during the production process. In the MEHR Method, microorganisms, oxygen, nutrients are put in a reservoir with used or contaminated oil with the purpose of enhancing oil recovery. The main objectives of these processes are to transform the hydrocarbon components found in oil. This will make oil to be more fluid and in viscid. This method can be used to clean environments, which have been contaminated with waste products produced by fossil fuels. Algae can be used to generate biofuels for industrial use. Although it is a ubiquitous organism, algae have been used in the pharmaceutical world and it can be applied in the production of clean energy. Algae that absorb carbon are a great potential and an alternative to the use of fossil fuels. One setback with the use of algae to produce biofuel, is the need of large systems (Earth Sciences Division, n.d). Sugar undergoes a series of chemical processes to produce ethanol. This process is referred to as fermentation. Ethanol and carbon dioxide are produced after microorganisms have fermented the sugar. Intellishare Environmental Company provides a soil remediation oxidizer, vacuum extractor and control systems. These packages can help in restoring the natural components and structure of soil in a contaminated area. In addition to that, the combination of the systems of this soil remediation method can slip through the cracks and help in treating the lower soil layers. Intellishare Environmental Companys soil remediation oxidizer, vacuum extractor and control system. Green Engineers recommend the use of Poly vinyl formamide is a less toxic chemical used in paper industries, water treatment plants, oil recovery, and industries used to design items for personal care. This chemical is not a neurotoxin even when ingested. Polyacrylamide, a chemical used in the same process is very toxic and can cause paralysis of the Central Nervous System when ingested. The waste produced is very expensive to clean up. In addition to that, CNS paralysis can lead to loss of lives, livelihoods, and increased dependency (Van Noordern, 2011). Green System Design Piping used in industries should slope downwards to reduce the amount of solvents used. Short and big pipes reduce the flow dram while using very low energy. Industries should not have dead-end sample points in order to reduce waste. Other green engineering changes that can be made to the industry include the use of efficient raw materials that are somehow refined. For instance, water used in refinery industries can be treated before being used to reduce sludge waste. Selectivity in rectors should also be increased to reduce the time taken to mix, catalyze. This can be enhanced by using highly efficient reactors, which take little residence time. Industries should use efficient separation processes. Conventionally, separate reaction and separation processes demanded the use of large amounts of solventss while consuming a lot of energy or pressure. Green technology has developed novel methods that allow products to separate by themselves due to their physical and chemical properties. The other method is through combined reaction and separation processes occupy little space, consume less energy, and are more efficient because it allows almost 99% conversion. Distillation and LLE processes can also be combined to increase efficiency and reduce waste. PET bottles can be recycled using simple processes. The colors of the bottles are sorted using spectroscopy since PET bottles have a characteristic green dye incorporated into their surface. The bottles are shredded and washed to remove labels. In the same water, HDPE and PET are separated through their different densities. The aluminum ring is removed using an electrostatic ring and the bottles can be reused. Efficiency Maximization Systems Processes, products should be designed to maximally utilize energy, mass, time and space in an efficient manner. Heat integration systems should be coupled to increase efficiency while reducing waste. For instance, in a cooling plant and heating plant, the systems can be interconnected in a way that steam from hot water is released to heat cold water. This reduces the loss of energy to the surroundings. According to La Chateliers principle, if stress is applied to a system, the system reacts by offsetting itself to relive the applied pressure. In industrial terms, stress can be defined as any factor that upsets the concentration gradient, for instance, temperature and pressure. Increase of an input into a production system will lead to an increase in the generation of an output. This design aims to minimize the quantities of resources used while getting a good amount of output. In this context, manufacturing systems can be based on timely manufacturing. Timely manufacturing refers to the production of goods to meet the consumers quantity, quality and in their time of demand. This method implies that the resources, equipment, and labor are available when there is a demand created by the consumers. This is a form of a planned manufacturing system that does manages waste, overproduction, reduces waiting time, inputs, and outputs. This enables that industries have minimized excess while meeting the needs of their consumers. Chlorination of water is simple and makes millions of water safe within a short time. This method spends little energy over water treatment plants that use unnecessary resources like heat, power, and labor. Green engineering advocates for methods like express metal deposition over metal casting because the waste produced by the earlier method is little. Reduction of Complexity The use of natural systems should not be avoided in the manufacturing process. The higher the complexity involved in making a product, the lower the chances of recycling the material. Products should be made in a way that they are durable rather than those ones that have a very long shelf life. If production processes target immortality, there is a high risk of the products to the environment and many potential hazards to the human life. For instance, eco-fill a food grade input is constituted from water and starch. It can be used in industrial production, very durable and exerts little burden to the environment because it is mortal. The other method that eliminates environmental hazards is the use of polylactic acid to manufacture fibers and plastics over polyacrylic acid, which is non-biodegradable (Center for Green Energy and Green Engineering, 2011). In conclusion, green engineering has taken a step in redesigning the production processes to eliminate waste and environmental pollution. This form of engineering aims at renewable products and services that pose a minimal or no risk to the environment and other life forms. Products that are designed with components that can be reused reduce waste in the environment. All companies should embrace these methods to reduce the hazards and risks posed to the environment because prevention of dumping is less expensive than cleaning up. Buy custom The Role of Chemical Engineering essay

Analysis of Public Speech Given by President George W. Bush essays

Analysis of Public Speech Given by President George W. Bush essays The following is an analysis of a speech given by the President of the United States, President George W. Bush on October 7, 2002. The speech was entitled President Bush Outlines Iraqi Threat and was presented at the Cincinnati Museum Center at Cincinnati Union Terminal, Cincinnati, Ohio. In the first instance this important speech is a characterized by a calm argumentative tone and logical persuasion. The central theme of the speech deals with the threat that Iraq poses to the United States and to the world in general. Besides the clear and calm but decisive tone that the speaker uses to convey his massage, the content of the speech is intended to draw attention and to emphasize the serious nature of the subject matter. For example, the speech takes a very broad and general view from the beginning when the President states that he intends to discuss, ... a great threat to peace. (President Bush Outlines Iraqi Threat) The use of wide-ranging and evocative words like threat and peace places the speech at a universal level which involves all people and is not localized. By so doing the speaker draws the audience attention immediately to what is obviously a crucial issue. The speaker then builds his argument and states clearly the reasons for the threat that Iraq poses. The argumentative and persuasive nature of the speech is enhanced by continual references to logical factors and their consequences. The speaker is careful to point out that the present crisis has arisen directly as a result of the actions and intentions of the Iraqi regime and is not a result of aggression on the part of the United States. The tone is consistently assertive and condemnatory and uses reason to support the central points. The speaker employs various techniques to bolster and enforce these main points and to make sure that there can be no doubt in the minds of audience as to his sincerity and ...

50 Great Topics for a Process Analysis Essay

50 Great Topics for a Process Analysis Essay If youve ever read an instruction manual or written out a set of directions, then you know what a process analysis essay is. This form of composition is often used in the field of technical writing, where complex systems need to be clearly explained in a logical, orderly fashion. As such, process analyses can be very detailed and sometimes quite long. Process analysis  writing is more than just a set of simple instructions. As a writer, you must go beyond merely identifying the steps involved and examine that process with an analytical eye. This analysis requires expertise- if not firsthand, then from research. Your topic needs to be focused, usually how to do one specific thing, and written in a clear, straightforward tone that readers can follow easily.   Tips for Writing a Process Analysis Essay When developing a paragraph, essay, or speech through process analysis, keep these tips in mind:Be sure to include all steps and arrange them in chronological order.Explain why each step is necessary, and include warnings where appropriate.Define any terms with which your readers may not be familiar.Offer clear descriptions ​of any tools or materials needed to carry out the process.Provide your readers with a way of determining whether the process has been carried out successfully or not. 50  Topic Suggestions: Process Analysis You shouldnt find it difficult to follow the guidelines above if youve chosen a topic that you know quite well. These 50 prompts are meant to help you discover that topic. How to mow your lawnHow to win at Texas hold emHow to lose weight without losing your mindHow to find the perfect roommateHow to get rid of a roommate- without committing a crimeHow to succeed in (or flunk out of) collegeHow to pitch a knuckleballHow to plan the perfect partyHow to survive a night of babysittingHow to pitch a tent in the rainHow to housebreak your dogHow to kick a bad habitHow to overcome insomniaHow to stay sober on a Saturday nightHow to rent your first apartmentHow to avoid a nervous breakdown during examsHow to enjoy the weekend for under $20How to make the perfect browniesHow to keep peace with a spouse or a roommateHow to bathe a catHow to complain effectivelyHow to survive a recessionHow to toilet train a babyHow to develop self-confidenceHow to use Twitter sensibly and effectivelyHow to wash a sweaterHow to build a great music collection- cheaply and legallyHow to get along with an instructor without sucking upHow to give yourself a haircutHow to plan the per fect class schedule How to apply the Heimlich maneuverHow to end a relationshipHow to select the best portable media playerHow to take decent photographs with your cell phoneHow to quit smokingHow to survive without a carHow to make the perfect cup of coffee or teaHow to save money while saving the environmentHow to build a great sandcastleHow to edit a videoHow to make (and keep) friends on FacebookHow to insert a contact lensHow teachers make up examsHow parents (or children) make us feel guiltyHow an iPod worksHow ice cream is madeHow a cell phone takes picturesHow a magician saws a woman in halfHow a pocket calculator worksHow to choose a major

Blog Response and write your own blog Essay Example | Topics and Well Written Essays - 750 words

Blog Response and write your own blog - Essay Example The advert correlates to the cultural notion that physical fitness, deep voice, and aggressive action are attributes of a man. Moreover, any man who lacks these qualities is shown as feminine. On the other hand, it is depicted that women in the society prefers men with maximum masculinity. This is because, based on their perception, such a man will be able to take care of women needs. This makes them admire men who have well developed figure. However, this post deserves criticism. This is because it depicts men who lacks such masculinities cannot have power, offer protection to women, and hence this is shown to be in contradiction to American culture. In reality, this is not true because being a real man is not based on outside appearance. The real man is defined by behaviour and instinct. Moreover, it is negatively depicting women as only attracted by physical well-being towards men. This is not true as women are also attracted by other attributes towards women. In fact, violent men are usually unable to sustain a formidable relationship. Part 2 The film ‘Dangerous Minds’ is a representation of what is covered with many Hollywood movies. The film depicts the worse state of education for prone kids who face the challenges of poverty, violence, crime, and violence in the inner cities of United States. The teacher also struggles to content with the students. Initially, the film depicts Whiteness as a true reflection of rationality. Moreover, the film shows Whiteness as a cultural standard in the changing society based on the racial composition. On the other hand, the other part of the film acts as an encouragement to challenged teenagers who had given up on schooling as an alternative to their wretched lives. In the film, the purpose of schooling is intermixed with the issue of racial difference within the area of urban public schools. Therefore, the film relies on race factor as an organizing concept to develop its narrative structure and pass throu gh the message. Moreover, both the Black and Hispanic teenagers lay a foundation for developing pedagogical classroom interaction. In this context, Whiteness as espoused by the teacher authority manages to overcome racially coded images of disorder, chaos, and fear. This is seen through the conflicts that exist between teacher and students in the first scenes of the film. This is a classical example of non- assimilation feelings between whites and non-whites. Moreover, the innocence of the teacher is highlighted in the first scene of the film. She leaves a middle-class culture in the white community to teach in uncontrollable environment. This is meant to provide white American with the notion that the disorder is a thing of other culture. The negative perception on the people of colour is further highlighted as she refers to them as â€Å"rejects from hell†. Therefore, the opening scenes serve to represent Black and Hispanic kids with the culture of violence and unruliness. On the other hand, Whiteness as depicted by LouAnne Johnson is under threat. This is because it is unsafe and under siege. Moreover, Whiteness is shown through the character of the teacher as the only option for teens to move beyond the setting and nature of their cultural identities. This occurs as it is contrasted with the stereotypical portrayal of intellectually challenged and decadent Black and Hispanic kids. Therefore, Whiteness is thus implied as a standard for